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FAQ
Why choose EDITGENE?
EDITGENE provides access to a comprehensive library of over 4,500 high-quality knockout (KO) cell lines, enabling researchers to save valuable time. Our custom gene knockout services are highly efficient, boasting a high positive rate while minimizing off-target effects. Clients also benefit from personalized, one-on-one support from a team of PhD experts from globally renowned institutions, ensuring top-tier service and results.
What is the difference between KO cell lines and gene knockout animal models?
KO cell lines are used for in vitro experiments, suitable for high-throughput screening and cellular studies, while gene knockout animal models are used for in vivo experiments to study gene functions within an entire organism and its interaction with the environment.
Why do researchers use KO cell lines?
Researchers use KO cell lines to investigate gene functions by observing the effects of gene deletion on cellular behavior. This helps in understanding the role of genes in various processes like cell growth, metabolism, and signal transduction. KO cell lines are vital for studying diseases like cancer, genetic disorders, and neurodegenerative diseases.
What is a KO cell line?
KO (Knockout) cell line is a cell line where a specific gene has been completely removed or rendered non-functional through gene editing technologies such as CRISPR-Cas9. These cell lines are critical for understanding gene functions and disease mechanisms.
Are KO cell lines applicable to all cell types?
KO cell lines can be applied to various cell types, including cancer cells, stem cells, and primary cells, but different cell types may have varying sensitivities to gene editing, and may vary among different cell types. In certain cell types, achieving gene knockout may require optimization of transfection conditions and selection of appropriate gene-editing tools.
What is gene overexpression?
Gene overexpression refers to using various techniques to significantly increase the expression level of a specific gene in cells or organisms. This is often achieved by introducing additional gene copies or using strong promoters to drive gene expression.
Why conduct gene overexpression?
Gene overexpression aids in studying the function of specific genes, revealing their role within the organism. It is also commonly used in drug screening, vaccine development, and protein production. For example, by overexpressing a therapeutic protein, researchers can evaluate its efficacy in disease models.
为什么研究人员要使用KO细胞系？
研究人员使用KO细胞系来解析基因功能，因为敲除基因能够揭示该基因在细胞生长、分化、代谢、信号传导等过程中的具体作用。通过比较敲除基因的细胞与正常细胞的差异，科学家可以深入了解基因在疾病中的作用，进而开发新的治疗方法。例如，KO细胞系在癌症、遗传病和神经退行性疾病的研究中具有重要应用。
KO细胞系是否适合所有类型的基因？
虽然KO细胞系技术强大，但并非所有基因都适合敲除。某些基因的敲除可能导致细胞死亡或严重的生理功能障碍，特别是那些对细胞存活至关重要的基因。在这种情况下，可以选择条件性敲除或基因敲降（如RNAi）来研究这些基因的功能。
如何选择全基因组或亚基因组CRISPR文库？
CRISPR文库类型可分为全基因组文库和亚基因组文库。如果目标是进行全基因组范围内的筛选，那么全基因组文库是最佳选择。这类文库通常包含针对整个基因组的sgRNA。如果研究目标有偏向性，如只关注特定基因家族或特定信号通路，那么可以选择亚基因组文库，以减少不必要的筛选工作量和成本。
iPSC如何帮助我们理解复杂疾病？
从患者分离出体细胞并重编程为iPCS，然后诱导分化为特定的细胞类型，用于创建疾病模型。这些模型可以帮助研究人员研究复杂疾病的发病机制，揭示疾病相关的基因和分子通路，从而推动新的治疗方法的开发。通过分析这些细胞，科学家可以观察到疾病在细胞水平上的变化，提供了疾病研究的新视角。
什么是单克隆筛选，为什么它在基因编辑研究中如此重要？
单克隆筛选是从混合细胞池中分选出单个克隆，并由该克隆培养扩增成细胞系。单克隆筛选保证了使用的细胞系是来源于同一个细胞，并且确保了遗传背景的高度一致。在细胞被基因编辑或基因修饰后，初始细胞池的每个细胞的遗传背景的差异是巨大的，这样的细胞池用于后续的实验，实验结果无疑是不准确的。通过单克隆筛选，可以获得遗传背景一致、具有稳定基因编辑的细胞群体，监测到的表型变化才是稳定准确的。
什么是KO细胞系？
KO细胞系（Knockout Cell Line）是通过基因编辑技术（如CRISPR-Cas9）敲除或删除特定基因的细胞系。这意味着目标基因被完全移除或失去功能，使研究人员能够观察该基因缺失对细胞行为和生物学过程的影响。这种细胞系在生物医学研究中非常重要，因为它们提供了直接了解基因功能和疾病机制的实验工具。
在临床应用中，iPSCs的潜在应用有哪些？
iPSC在临床中具有广泛的应用潜力，包括用于细胞治疗（如治疗糖尿病或心脏病）、组织工程（如开发人工皮肤或肝脏组织）、以及个性化药物筛选（如根据患者特定的细胞反应来选择最佳治疗方案）。这些应用方向可能会改变治疗的方式，提供更有效和个性化的医疗服务。
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